The tumor-suppressive effects of human chorionic gonadotropin (hCG) against human breast cancer cells were examined. In cell viability assays, hCG inhibited the growth of three human breast cancer cell lines (estrogen receptor (ER)-positive KPL-1 and MCF-7, and ER-negative MKL-F cells), and the growth inhibition activity of hCG was most pronounced against KPL-1 cells (luteinizing hormone/chorio...

We report an experimental measurement of the temporal dependence of the area Aus in a twodimensional soap froth which has not been swept by the passage of soap films up to time t, as the froth coarsens from an initial time t0 within the scaling regime. We find Aus scales with the mean cell area kAl as Aus ~ kAl 0 , with a first-passage exponent u0 ­ 1.16 6 0.02; and for the average perimeter kP...

The effects of mead acid (MA; 5,8,11-eicosatrienoic acid) on the suppression of breast cancer cell growth and metastasis were examined in vitro and in vivo by using the KPL-1 human breast cancer cell line. MA suppressed KPL-1 cell growth in culture with an IC50 value of 214.2 µM (65.7 µg/ml) for 72 h, and MA significantly suppressed transplanted KPL-1 tumor growth (tumor volume and tumor weight...

PURPOSE To estimate the rate constant for pyruvate to lactate conversion in tumours in response to a hypoxic challenge, using hyperpolarised (13)C1-pyruvate and magnetic resonance spectroscopy. METHODS AND MATERIALS Hypoxic inspired gas was used to manipulate rat P22 fibrosarcoma oxygen tension (pO2), confirmed by luminescence decay of oxygen-sensitive probes. Hyperpolarised (13)C1-pyruvate w...

In many solid tumors, overexpression of human epidermal growth factor receptors (e.g., HER1/EGFR and HER2) correlates with poor prognosis. Erlotinib (Tarceva) is a potent HER1/EGFR tyrosine kinase inhibitor. Pertuzumab (Omnitarg), a novel HER2-specific, recombinant, humanized monoclonal antibody, prevents heterodimerization of HER2 with other HERs. Both mechanisms disrupt signaling pathways, re...

The background problem in this research is that the 2015 Informatics Engineering Study Program students have low interest teaching profession. This study aims to: (1) describe Field Practice Study, self-efficacy, pedagogic competence, and being a teacher; (2) revealing partial effect of competence on becoming teacher undergraduate Education Faculty Engineering, State University Malang; (3) simu...

The inactive MoFe protein of nitrogenase, NifB-Kp 1, from two distinct niB mutants of Klebsiella pneumoniae, Kp5058 (a nifB point mutant) and UNF1718 (a nijB, nifJ double mutant) has been purified and characterized. NifB-Kpl can be activated by reaction with the iron-molybdenum cofactor, FeMoco, extracted from active MoFe protein. NifB-Kpl purified from either source had similar properties and ...

Antibodies are a key element in immunoassays They are the driving force behind specificity and sensitivity. Affinity purification of antiserum yields antibodies with the ultimate in specificity and sensitivity. It eliminates all other potentially interfering serum proteins and antibodies that will increase the background and cause cross-reactivity. It leaves behind a population of antibodies th...

Maassen, N., M. Foerster, and H. Mairbäurl. Red blood cells do not contribute to removal of K1 released from exhaustively working forearm muscle. J. Appl. Physiol. 85(1): 326–332, 1998.—K1 released from exercising muscle via K1 channels needs to be removed from the interstitium into the blood to maintain high muscle cell membrane potential and allow normal muscle contractility. Uptake by red bl...

PURPOSE Resistance to anti-HER2 monoclonal antibody trastuzumab is a relevant issue in breast cancer patients. Among the mechanisms implicated in trastuzumab resistance, increasing evidence supports a role of tumor microenvironment. We previously found that a novel toll-like receptor 9 agonist, referred to as immune modulatory oligonucleotide (IMO) and currently under clinical investigation, ac...